human normal liver cell line lo2  (ATCC)

Journal: Journal of Cancer

Article Title: Prognostic and Immune Landscape Analysis of Ubiquitination-related Genes in Hepatocellular Carcinoma: Based on Bulk and Single-cell RNA Sequencing Data

doi: 10.7150/jca.93425

Figure Lengend Snippet: Validation of UbRGs expression was based on HPA database and experiments. (A) The expression profiles of UbRGs in normal and tumor tissues were retrieved from the TCGA database, and the immunohistochemistry images of UbRGs were obtained from the HPA database. (B) RT-qPCR was used to measure the expression levels of BOP1, CDC20, and UBE2S genes in the normal liver cell line (LO2) as well as in liver cancer cell lines (HepG2, Huh7, MHCC97H). (C) Western blotting (WB) was performed to detect the protein levels of BOP1, CDC20, and UBE2S in the normal liver cell line (LO2) as well as in liver cancer cell lines (HepG2, Huh7, MHCC97H). (D) The bar graph illustrates the expression differences of UbRGs among different cell types. (E) Immunofluorescence was used to determine the subcellular localization of UbRGs in tumor cells.

Article Snippet: Human liver cancer cell lines (HepG2, Huh7, and MHCC97H) were purchased from ServiceBio, and human normal liver cell lines (LO2) were purchased from iCell Bioscience.

Techniques: Biomarker Discovery, Expressing, Immunohistochemistry, Quantitative RT-PCR, Western Blot, Immunofluorescence

Journal: Discovery Medicine

Article Title: Artesunate Inhibits Lipid Accumulation and Inflammation by Regulating the NLRP3 Inflammasome in Nonalcoholic Fatty Liver Disease

doi: 10.24976/discov.med.202436181.36

Figure Lengend Snippet: Fig. 1. Effect of artesunate (ART) on cell viability in LO2 cells after oleic acid (OA) treatment. (A) Effect of OA at different

Article Snippet: Human normal liver cells LO2 (TongPai, Shanghai, China) were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (CM10041, MACGENE, Beijing, China) supplemented with 10% fetal bovine serum (FBS, FCS500, EXcellBio, Shanghai, China) and 100 U/mL penicillin-streptomycin (P1400, Solarbio, Beijing, China).

Techniques:

Journal: Discovery Medicine

Article Title: Artesunate Inhibits Lipid Accumulation and Inflammation by Regulating the NLRP3 Inflammasome in Nonalcoholic Fatty Liver Disease

doi: 10.24976/discov.med.202436181.36

Figure Lengend Snippet: Fig. 2. ART restrained lipid accumulation in LO2 cells after OA treatment. (A) Lipid accumulation was demonstrated by oil red O staining (n = 3) (scale bar: 50 µm). (B) Effect of ART on triglycerides (TG) in LO2 cells after OA treatment (n = 3). (C) Effect of ART

Article Snippet: Human normal liver cells LO2 (TongPai, Shanghai, China) were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (CM10041, MACGENE, Beijing, China) supplemented with 10% fetal bovine serum (FBS, FCS500, EXcellBio, Shanghai, China) and 100 U/mL penicillin-streptomycin (P1400, Solarbio, Beijing, China).

Techniques: Staining

Journal: Discovery Medicine

Article Title: Artesunate Inhibits Lipid Accumulation and Inflammation by Regulating the NLRP3 Inflammasome in Nonalcoholic Fatty Liver Disease

doi: 10.24976/discov.med.202436181.36

Figure Lengend Snippet: Fig. 3. ART decreased the secretion of pro-inflammatory factors in OA treated LO2 cells. (A) Effect of ART on tumor necrosis

Article Snippet: Human normal liver cells LO2 (TongPai, Shanghai, China) were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (CM10041, MACGENE, Beijing, China) supplemented with 10% fetal bovine serum (FBS, FCS500, EXcellBio, Shanghai, China) and 100 U/mL penicillin-streptomycin (P1400, Solarbio, Beijing, China).

Techniques: